Anticorps Monoclonal anti-NFATC3

NFATC3 Monoclonal Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C1H8

N° de cat : 66924-1-PBS

Synonymes

1C1H8, NF AT4, NF ATc3, NFAT4, NF-AT4

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Galerie de données de validation

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  WB analysis using 66924-1-Ig (same clone as 66924-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66924-1-Ig (NFATC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66924-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50022-1

  Sandwich ELISA standard curve of MP50022-1, NFATC3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66924-2-PBS. Detection antibody: HRP-conjugated 66924-1-PBS. Standard: Ag27306. Range: 0.625-20 ng/mL.

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  IHC staining of human thymus using 66924-1-Ig (same clone as 66924-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 66924-1-Ig (NFATC3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66924-1-PBS in a different storage buffer formulation.

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  IHC staining of human thymus using 66924-1-Ig (same clone as 66924-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 66924-1-Ig (NFATC3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66924-1-PBS in a different storage buffer formulation.

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  IF Staining of SKOV-3 using 66924-1-Ig (same clone as 66924-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using NFATC3 antibody (66924-1-Ig, Clone: 1C1H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66924-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66924-1-PBS cible NFATC3 dans les applications de WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NFATC3 Protéine recombinante Ag27306
• Nom complet: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
• Masse moléculaire calculée: 116 kDa
• Poids moléculaire observé: 160 kDa
• Numéro d’acquisition GenBank: BC001050
• Symbole du gène: NFATC3
• Identification du gène (NCBI): 4775
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NFAT (nuclear factors of activated T cells) proteins are a family of transcription factors originally identified as mediators of activation of cytokine genes in response to antigenic stimulation of T cells. NFAT proteins also play varied roles in cells outside of the immune system [PMID:11877454]. NFATc3 has specifically been implicated in vasculature development, regulation of smooth muscle contractile phenotype, and modulation of vascular smooth muscle contractility [PMID:11439183,17148444]. The calculated molecular weight of NFATC3 is 115 kDa, but the post-modified protein is about 130-170 kDa (PMID: 15728531, PMID: 15857835)

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