Anticorps Polyclonal de lapin anti-NFIB

• Phare
• Validé par KD/KO

NFIB Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29898-1-PBS

Synonymes

NF1-B, NF1 B, NF I/B, HMGIC/NFIB, CTF

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Galerie de données de validation

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  WB analysis using 29898-1-AP (same clone as 29898-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29898-1-AP (NFIB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29898-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 29898-1-AP (same clone as 29898-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 29898-1-AP (NFIB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29898-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 29898-1-AP (same clone as 29898-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NFIB antibody (29898-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29898-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 29898-1-AP (same clone as 29898-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NFIB antibody (29898-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29898-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 29898-1-AP (same clone as 29898-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human NFIB (29898-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 29898-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29898-1-PBS cible NFIB dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NFIB Protéine recombinante Ag31946
• Nom complet: nuclear factor I/B
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 45-65 kDa
• Numéro d’acquisition GenBank: BC001283
• Symbole du gène: NFIB
• Identification du gène (NCBI): 4781
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NFIB, also named as Nuclear factor 1, is a 420 amino acid protein, which belongs to the CTF/NF-I family and may bind DNA as a homodimer. NFIB recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. NFIB also exists in multiple isoforms in prostate cancer cells, with average molecular weights of 62 kDa, 57 kDa, and 49 kDa (PMID: 32692871).

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