Anticorps Monoclonal anti-NFIL3

NFIL3 Monoclonal Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

WB, IF, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

2F4B4

N° de cat : 66368-1-Ig

Synonymes

Interleukin-3-binding protein 1, Interleukin-3 promoter transcriptional activator, IL3BP1, E4BP4, E4 promoter-binding protein 4

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Galerie de données de validation

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  WB analysis of human skeletal muscle using 66368-1-Ig

  Western blot analysis of NFIL3 in various cell lines and tissues using Proteintech antibody 66368-1-Ig at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of NK92 using 66368-1-Ig

  1X10^6 NK92 were intracellularly stained with 0.4 ug Anti-Human NFIL3 (66368-1-Ig, Clone:2F4B4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique humain
• Résultats positifs en FC (Intra): NK92, NK92 cells

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

66368-1-Ig cible NFIL3 dans les applications de WB, IF, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NFIL3 Protéine recombinante Ag2380
• Nom complet: nuclear factor, interleukin 3 regulated
• Masse moléculaire calculée: 462 aa, 51 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC008197
• Symbole du gène: NFIL3
• Identification du gène (NCBI): 4783
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NFIL3, also named E4BP4 and IL3BP1, belongs to the bZIP family and NFIL3 subfamily. It represses transcription from promoters with activating transcription factor (ATF) sites. NFIL3 activates transcription from the interleukin-3 promoter in T-cells. It competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). It is a component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. NFIL3 protects pro-B cells from programmed cell death. It is a key regulator of TH2 responses(PMID:21499227).

Publications

Species	Application	Title
human	IF	Cell Death Dis; Tubular CD44 plays a key role in aggravating AKI through NF-κB p65-mediated mitochondrial dysfunction; Authors - Jiewu Huang

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