Anticorps Monoclonal anti-NFKB1,p105,p50

NFKB1,p105,p50 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

2G1E3

N° de cat : 66992-1-Ig

Synonymes

DKFZp686C01211, DNA binding factor KBF1, EBP 1, KBF1, NF kappa B, NFKB p105, NFKB p50, NFKB1, NFKB1,p105, NFKB1,p105,p50, p105, p50

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Galerie de données de validation

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  WB analysis using 66992-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66992-1-Ig (NFKB1,p105,p50 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66992-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66992-1-Ig (NFKB1,p105,p50 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66992-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66992-1-Ig (NFKB1,p105,p50 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66992-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NFKB1,p105,p50 antibody (66992-1-Ig, Clone: 2G1E3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HepG2 using 66992-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NFKB1,p105,p50 (66992-1-Ig, Clone:2G1E3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HeLa, cellules Jurkat, cellules K-562, cellules THP-1
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF): IF : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

66992-1-Ig cible NFKB1,p105,p50 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NFKB1,p105,p50 Protéine recombinante Ag5832
• Nom complet: nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
• Masse moléculaire calculée: 105 kDa
• Poids moléculaire observé: 50 kDa, 105 kDa
• Numéro d’acquisition GenBank: BC051765
• Symbole du gène: NFKB1
• Identification du gène (NCBI): 4790
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NFkB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFkB is activated by various intra- and extracellular stimuli such as cytokines, oxidant free radicals, ultraviolet irradiation, and bacterial or viral products. NFkB is a family of transcription factors that consists of homo- and heterodimers of NFkB1/p50 and RelA/p65 subunits, and controls a variety of cellular events including development and immune responses. All members share a conserved amino terminus domain that includes dimerization, nuclear localization, and DNA binding regions, and a carboxy terminal transactivation domain. Serines 529 and 536 in the transactivation domain of RelA/p65 are phosphorylated in response to several stimuli including phorbol ester, IL1 alpha and TNF alpha as mediated by IkB kinase and p38 MAPK. Phosphorylation of serines 529 and 536 is critical for RelA/p65 transcriptional activity. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. This antibody can bind both p105 and p50 isoforms of NFKB1.

Publications

Species	Application	Title
mouse	IF	J Ethnopharmacol; Sub-chronic exposure to realgar induces liver injury via upregulating the TXNIP/NLRP3 pathway and disturbing bile acid homeostasis in mice; Authors - Aihong Li
mouse	IF,WB	Cell Commun Signal; Semaphorin 7A interacts with nuclear factor NF-kappa-B p105 via integrin β1 and mediates inflammation; Authors - Xuan Li
mouse	WB,IF	Adv Sci (Weinh); NKRF in Cardiac Fibroblasts Protects against Cardiac Remodeling Post-Myocardial Infarction via Human Antigen R; Authors - Chenghu Guo

Produits associés

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011