Anticorps Monoclonal anti-NFKB1,p105,p50

NFKB1,p105,p50 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2G1E3

N° de cat : 66992-1-PBS

Synonymes

NFKB1, KBF1, EBP-1, EBP 1, DNA-binding factor KBF1

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Galerie de données de validation

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  WB analysis using 66992-1-Ig (same clone as 66992-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66992-1-Ig (NFKB1,p105,p50 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66992-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 66992-1-Ig (same clone as 66992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66992-1-Ig (NFKB1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66992-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 66992-1-Ig (same clone as 66992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66992-1-Ig (NFKB1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66992-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66992-1-Ig (same clone as 66992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66992-1-Ig (NFKB1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66992-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66992-1-Ig (same clone as 66992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66992-1-Ig (NFKB1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66992-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66992-1-Ig (same clone as 66992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66992-1-Ig (NFKB1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66992-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66992-1-Ig (same clone as 66992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66992-1-Ig (NFKB1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66992-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 66992-1-Ig (same clone as 66992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66992-1-Ig (NFKB1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66992-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 66992-1-Ig (same clone as 66992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66992-1-Ig (NFKB1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66992-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66992-1-Ig (same clone as 66992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66992-1-Ig (NFKB1,p105,p50 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66992-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66992-1-Ig (same clone as 66992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66992-1-Ig (NFKB1,p105,p50 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66992-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66992-1-Ig (same clone as 66992-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NFKB1,p105,p50 antibody (66992-1-Ig, Clone: 2G1E3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66992-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66992-1-Ig (same clone as 66992-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NFKB1,p105,p50 (66992-1-Ig, Clone:2G1E3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66992-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51119-1

  Cytometric bead array standard curve of MP51119-1, NFKB1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66992-2-PBS. Detection antibody: 66992-1-PBS. Standard:Ag5832. Range: 3.125-100 ng/mL.

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Informations sur le produit

66992-1-PBS cible NFKB1,p105,p50 dans les applications de WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NFKB1,p105,p50 Protéine recombinante Ag5832
• Nom complet: nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
• Masse moléculaire calculée: 105 kDa
• Poids moléculaire observé: 50 kDa, 105 kDa
• Numéro d’acquisition GenBank: BC051765
• Symbole du gène: NFKB1
• Identification du gène (NCBI): 4790
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NFkB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFkB is activated by various intra- and extracellular stimuli such as cytokines, oxidant free radicals, ultraviolet irradiation, and bacterial or viral products. NFkB is a family of transcription factors that consists of homo- and heterodimers of NFkB1/p50 and RelA/p65 subunits, and controls a variety of cellular events including development and immune responses. All members share a conserved amino terminus domain that includes dimerization, nuclear localization, and DNA binding regions, and a carboxy terminal transactivation domain. Serines 529 and 536 in the transactivation domain of RelA/p65 are phosphorylated in response to several stimuli including phorbol ester, IL1 alpha and TNF alpha as mediated by IkB kinase and p38 MAPK. Phosphorylation of serines 529 and 536 is critical for RelA/p65 transcriptional activity. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. This antibody can bind both p105 and p50 isoforms of NFKB1.

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