Anticorps Monoclonal anti-NFKB2

• Phare
• Validé par KD/KO

NFKB2 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

6A10E9

N° de cat : 66920-1-PBS

Synonymes

6A10E9, DNA binding factor KBF2, DNA-binding factor KBF2, H2TF1, LYT 10

---

Galerie de données de validation

• 

  WB analysis using 66920-1-Ig (same clone as 66920-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66920-1-Ig (NFKB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66920-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66920-1-Ig (same clone as 66920-1-PBS)

  N/A This data was developed using the same antibody clone with 66920-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66920-1-Ig (same clone as 66920-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66920-1-Ig (NFKB2 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66920-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66920-1-Ig (same clone as 66920-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66920-1-Ig (NFKB2 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66920-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66920-1-Ig (same clone as 66920-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66920-1-Ig (NFKB2 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66920-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66920-1-Ig (same clone as 66920-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66920-1-Ig (NFKB2 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66920-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66920-1-PBS cible NFKB2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NFKB2 Protéine recombinante Ag28543
• Nom complet: nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC002844
• Symbole du gène: NFKB2
• Identification du gène (NCBI): 4791
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.

{{ptg:RelatedPrimaryAntibodies}}