Anticorps Recombinant de lapin anti-NFKB2,p100

NFKB2,p100 Recombinant Antibody for IF/ICC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

241126F1

N° de cat : CL488-84022-6

Synonymes

NFKB2, LYT10, LYT 10, H2TF1, DNA-binding factor KBF2

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Galerie de données de validation

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  IF Staining of U2OS using CL488-84022-6

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 NFKB2,p100 antibody (CL488-84022-6, Clone: 241126F1 ) at dilution of 1:200.

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Applications testées

• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-84022-6 cible NFKB2,p100 dans les applications de IF/ICC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NFKB2,p100 Protéine recombinante Ag0662
• Nom complet: nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC002844
• Symbole du gène: NFKB2
• Identification du gène (NCBI): 4791
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. This antibody can bind p100 isoform of NFKB2 spefically, but not p52 isoform.

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