Anticorps Polyclonal de lapin anti-NFKBIZ

• Phare
• Validé par KD/KO

NFKBIZ Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14014-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis of HeLa using 14014-1-AP (same clone as 14014-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14014-1-AP (NFKBIZ antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14014-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14014-1-AP (same clone as 14014-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14014-1-AP (NFKBIZ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14014-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 14014-1-AP (same clone as 14014-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14014-1-AP (NFKBIZ Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 14014-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 14014-1-AP (same clone as 14014-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14014-1-AP (NFKBIZ Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14014-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 14014-1-AP (same clone as 14014-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NFKBIZ antibody (14014-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14014-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

14014-1-PBS cible NFKBIZ dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NFKBIZ Protéine recombinante Ag5120
• Nom complet: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta
• Masse moléculaire calculée: 78 kDa
• Poids moléculaire observé: 78 kDa, 64 kDa
• Numéro d’acquisition GenBank: BC060800
• Symbole du gène: NFKBIZ
• Identification du gène (NCBI): 64332
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NFKBIZ, also named as NF-kappa-B inhibitor zeta, is a 718 amino acid protein, which contains 7 ANK repeats and is expressed at high levels in peripheral blood leukocytes and lung, at moderate levels in liver, placenta, and at low levels in spleen, kidney, skeletal muscle and heart. NFKBIZ exists as three isoforms and molecular weight of isoform three is a 64 kDa. The isoform one is 78 kDa protein and isoform two is a 68 kDa protein. NFKBIZ is involved in regulation of NF-kappa-B transcription factor complexes. It Inhibits NF-kappa-B activity without affecting its nuclear translocation upon stimulation.

{{ptg:RelatedPrimaryAntibodies}}