Anticorps Monoclonal anti-NFS1

• Phare
• Validé par KD/KO

NFS1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

3A10A7

N° de cat : 67021-1-Ig

Synonymes

3A10A7, Cysteine desulfurase, EC:2.8.1.7, HUSSY 08, HUSSY-08

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Galerie de données de validation

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  WB analysis of HSC-T6 using 67021-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67021-1-Ig

  WB result of NFS1 antibody (67021-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NFS1 transfected HepG2 cells.

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  WB analysis of NIH/3T3 using 67021-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 67021-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67021-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67021-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67021-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HSC-T6, cellules HEK-293, cellules HeLa, cellules HepG2, cellules NIH/3T3, cellules RAW 264.7

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

67021-1-Ig cible NFS1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NFS1 Protéine recombinante Ag8017
• Nom complet: NFS1 nitrogen fixation 1 homolog (S. cerevisiae)
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC018471
• Symbole du gène: NFS1
• Identification du gène (NCBI): 9054
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NFS1(nitrogen fixation 1 homolog) is also named as NIFS, HUSSY-08 and belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. The protein has been identified as a pyridoxal phosphate-containing homodimer that catalyzes the formation of equimolar amounts of elemental sulfur and L-alanine from the substrate, L-cysteine. It is reported that NFS1 is also able to catalyze the removal of selenium from selenocysteine, a mechanism similar to the L-cysteine reaction was postulated(PMID:9812986). It has 2 isoforms produced by alternative initiation.

Publications

Species	Application	Title
human,rat	WB,IHC,IF	Free Radic Biol Med; GLS1-mediated glutamine metabolism mitigates oxidative stress-induced matrix degradation, ferroptosis, and senescence in nucleus pulposus cells by modulating Fe2+ homeostasis; Authors - Jiajun Wu
human	WB	Redox Biol; Histone lactylation drives liver cancer metastasis by facilitating NSF1-mediated ferroptosis resistance after microwave ablation; Authors - Jiayan Huang ; KD Validated

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