Anticorps Polyclonal de lapin anti-NFU1

NFU1 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25598-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis of mouse cerebellum using 25598-1-AP (same clone as 25598-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 25598-1-AP (NFU1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25598-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 25598-1-AP (same clone as 25598-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25598-1-AP (NFU1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 25598-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse cerebellum using 25598-1-AP (same clone as 25598-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 25598-1-AP (NFU1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 25598-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 25598-1-AP (same clone as 25598-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25598-1-AP (NFU1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 25598-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 25598-1-AP (same clone as 25598-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25598-1-AP (NFU1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25598-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 25598-1-AP (same clone as 25598-1-PBS)

  IP result of anti-NFU1 (IP:25598-1-AP, 4ug; Detection:25598-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 25598-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 25598-1-AP (same clone as 25598-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 25598-1-AP (NFU1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25598-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

25598-1-PBS cible NFU1 dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NFU1 Protéine recombinante Ag22343
• Nom complet: NFU1 iron-sulfur cluster scaffold homolog (S. cerevisiae)
• Masse moléculaire calculée: 254 aa, 28 kDa
• Poids moléculaire observé: 23-28 kDa
• Numéro d’acquisition GenBank: BC113692
• Symbole du gène: NFU1
• Identification du gène (NCBI): 27247
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NFU1, also named as HIRA-interacting protein 5 or CGI-33, is a 254 amino acid protein, which belongs to the NifU family. NFU1 localizes in the Mitochondrion. NFU1 is widely expressed in adult tissues. NFU1, as an Iron-sulfur cluster scaffold protein, which can assemble clusters and deliver them to target proteins. It has an association with Multiple mitochondrial dysfunctions syndrome 1.

{{ptg:RelatedPrimaryAntibodies}}