Anticorps Polyclonal de lapin anti-Lipocalin-2/NGAL

• Phare
• Validé par KD/KO

Lipocalin-2/NGAL Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30700-1-PBS

Synonymes

LCN2, lipocalin 2, NGAL, 24p3, 25 kDa alpha-2-microglobulin-related subunit of MMP-9

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Galerie de données de validation

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  WB analysis using 30700-1-AP (same clone as 30700-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30700-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30700-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-29 using 30700-1-AP (same clone as 30700-1-PBS)

  WB result of NGAL antibody (30700-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NGAL transfected HT-29 cells. This data was developed using the same antibody clone with 30700-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 30700-1-AP (same clone as 30700-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30700-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30700-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 30700-1-AP (same clone as 30700-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30700-1-AP (LCN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30700-1-PBS in a different storage buffer formulation.

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  IHC staining of Human bowens disease using 30700-1-AP (same clone as 30700-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 30700-1-AP (NGAL antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30700-1-PBS in a different storage buffer formulation.

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  IF Staining of RAW 264.7 using 30700-1-AP (same clone as 30700-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using Lipocalin-2/NGAL antibody (30700-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30700-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30700-1-PBS cible Lipocalin-2/NGAL dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Lipocalin-2/NGAL Protéine recombinante Ag33369
• Nom complet: lipocalin 2
• Poids moléculaire observé: 22-25 kDa
• Symbole du gène: NGAL/LCN2
• Identification du gène (NCBI): 3934
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Lipocalin-2 (LCN-2) is a adipocytokine also referred to as neutrophil gelatinase-associated lipocalin (NGAL). Lipocalin-2 is a circulatory protein responsible for the transportation of small and hydrophobic molecules to target organs. Lipocalin-2 is used as a biomarker for acute and chronic renal injury. It is present in a large variety of cells including neutrophil, hepatocytes, lung, bone marrow, adipose tissue, macrophages, thymus, non-neoplastic breast duct, prostate, and renal cells. Different functions have been associated with Lipocalin-2, including antibacterial, anti-inflammatory, and protection against cell and tissue stress (PMID:34463264).

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