Anticorps Polyclonal de lapin anti-p75NTR

p75NTR Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

24

Avis (1)

N° de cat : 55014-1-AP

Synonymes

CD271, Gp80 LNGFR, NGF receptor, NGFR, p75, p75 ICD, p75(NTR), p75NTR, TNFRSF16

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Galerie de données de validation

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  WB analysis of mouse brain using 55014-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55014-1-AP (p75NTR antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 55014-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 55014-1-AP (p75NTR antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 55014-1-AP

  IP Result of anti-p75NTR (IP:55014-1-AP, 3ug; Detection:55014-1-AP 1:300) with mouse brain tissue lysate 5000ug.

• 

  IHC staining of mouse brain using 55014-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55014-1-AP (p75NTR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 55014-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55014-1-AP (p75NTR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate hyperplasia using 55014-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 55014-1-AP (p75NTR antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 55014-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 55014-1-AP (p75NTR antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 55014-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 55014-1-AP (p75NTR antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 55014-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 55014-1-AP (p75NTR antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of SH-SY5Y using 55014-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug p75NTR antibody (55014-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu d'hyperplasie de la prostate humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 12 publications below
• IHC: See 5 publications below

Informations sur le produit

55014-1-AP cible p75NTR dans les applications de WB, IP, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: nerve growth factor receptor (TNFR superfamily, member 16)
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: NM_002507
• Symbole du gène: NGFR
• Identification du gène (NCBI): 4804
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NGFR, also named as TNFRSF16, Gp80-LNGFR, p75 ICD and CD271, is a low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. It mediates cell survival as well as cell death of neural cells. The antibody is specific to NGFR.

Publications

Species	Application	Title
mouse		J Exp Med; CXCR4 regulates Plasmodium development in mouse and human hepatocytes.; Authors - Hironori Bando
mouse	WB	Int J Mol Sci; Direct Enhancement Effect of Hippocampal Cholinergic Neurostimulating Peptide on Cholinergic Activity in the Hippocampus; Authors - Yuta Madokoro
human	IHC	Int J Mol Sci; Early Clinical Outcomes of the First Commercialized Human Autologous Ex Vivo Cultivated Oral Mucosal Epithelial Cell Transplantation for Limbal Stem Cell Deficiency: Two Case Reports and Literature Review; Authors - Hiroshi Toshida
human,mouse	WB,IHC	Front Oncol; LMO1 Plays an Oncogenic Role in Human Glioma Associated With NF-kB Pathway.; Authors - Lei Gao
human	WB	Stem Cell Res Ther; NT3P75-2 gene-modified bone mesenchymal stem cells improve neurological function recovery in mouse TBI model.; Authors - Ke Wu
human		J Mol Med (Berl); MESP2 variants contribute to conotruncal heart defects by inhibiting cardiac neural crest cell proliferation.; Authors - Erge Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Renato (Verified Customer) (02-27-2019)

Produces 3 perfectly discerning bands, but one of which clearly identifiable as p75NTR as it shows at the expected molecular weight. Used as diluent 5% BSA.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Brain tissue

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