Anticorps Polyclonal de lapin anti-NHE1

NHE1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29761-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 29761-1-AP (same clone as 29761-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29761-1-AP (NHE1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29761-1-PBS in a different storage buffer formulation.

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  WB analysis using 29761-1-AP (same clone as 29761-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29761-1-AP (NHE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29761-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 29761-1-AP (same clone as 29761-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 29761-1-AP (NHE1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 29761-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 29761-1-AP (same clone as 29761-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 29761-1-AP (NHE1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 29761-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 29761-1-AP (same clone as 29761-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NHE1 antibody (29761-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29761-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29761-1-PBS cible NHE1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NHE1 Protéine recombinante Ag31192
• Nom complet: solute carrier family 9 (sodium/hydrogen exchanger), member 1
• Masse moléculaire calculée: 91 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: NM_003047
• Symbole du gène: NHE1
• Identification du gène (NCBI): 6548
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NHE1, also named as SLC9A1, encodes an Na+/H+ antiporter that is a membrane-bound enzyme involved in regulating pH of vertebrate cells. NHE1 regulates a number of cell behaviors, including adhesion, shape determination, migration, and proliferation (PMID:11807182). It is inhibited by the non-specific diuretic drug amiloride and activated by growth factors, mitogens, neurotransmitters, tumor promoters, and others (PMID: 2846238). NHE1 is expressed in heart where it contributes to cardiomyocyte pH homeostasis (PMID: 12832126, PMID: 23709596).

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