Anticorps Polyclonal de lapin anti-NHE3

NHE3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27190-1-PBS

Synonymes

SLC9A3, NHE-3

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Galerie de données de validation

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  WB analysis of mouse small intestine using 27190-1-AP (same clone as 27190-1-PBS)

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 27190-1-AP (NHE3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27190-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 27190-1-AP (same clone as 27190-1-PBS)

  IP result of anti-NHE3 (IP:27190-1-AP, 4ug; Detection:27190-1-AP 1:1000) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 27190-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 27190-1-AP (same clone as 27190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27190-1-AP (NHE3 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH9). Arrows show brush border. This data was developed using the same antibody clone with 27190-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 27190-1-AP (same clone as 27190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27190-1-AP (NHE3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27190-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27190-1-PBS cible NHE3 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NHE3 Protéine recombinante Ag25637
• Nom complet: solute carrier family 9 (sodium/hydrogen exchanger), member 3
• Masse moléculaire calculée: 834 aa, 93 kDa
• Poids moléculaire observé: 80-100 kDa
• Numéro d’acquisition GenBank: BC101669
• Symbole du gène: NHE3
• Identification du gène (NCBI): 6550
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SLC9A3, also known as NHE3, belongs to the sodium hydrogen exchange protein family (NHE) and is mainly responsible for regulating the concentration of sodium and hydrogen ions inside and outside the cell. SLC9A3 is a Major apical Na+/H+ exchanger in kidney and intestine, playing an important role in renal and intestine Na+ absorption and blood pressure regulation (PMID:24622516, 2635877). Mutations in SLC9A3 cause Congenital Sodium Diarrhea (PMID: 26358773, 31276831).

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