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Anticorps Polyclonal de lapin anti-NIPBL

NIPBL Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18792-1-PBS

Synonymes

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of HeLa cells using NIPBL Polyclonal antibody (18792-1-AP)

    WB analysis of HeLa using 18792-1-AP (same clone as 18792-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 18792-1-AP (NIPBL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18792-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NIPBL Polyclonal antibody (18792-1-AP)

    WB analysis of HeLa using 18792-1-AP (same clone as 18792-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 18792-1-AP (NIPBL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18792-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NIPBL Polyclonal antibody (18792-1-AP)

    IF Staining of HeLa using 18792-1-AP (same clone as 18792-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 18792-1-AP (NIPBL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18792-1-PBS in a different storage buffer formulation.

Informations sur le produit

18792-1-PBS cible NIPBL dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène NIPBL Protéine recombinante Ag5663
Nom complet Nipped-B homolog (Drosophila)
Masse moléculaire calculée 2804 aa, 316 kDa
Poids moléculaire observé 300 kDa
Numéro d’acquisition GenBankBC033847
Symbole du gène NIPBL
Identification du gène (NCBI) 25836
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NIPBL, also named as Nipped-B-like protein, is a 28-4 amino acid protein, which Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains and belongs to the SCC2/Nipped-B family. NIPBL probably plays a structural role in chromatin and Involve in sister chromatid cohesion, possibly by interacting with the cohesin complex. endogenous full length NIPBL which is estimated to be about 300kDa led us to believe that the NIPBL protein was deteriorated or lost by the standard protein extraction and blotting techniques.

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