Anticorps Recombinant de lapin anti-NKCC1/SLC12A2

NKCC1/SLC12A2 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250829D8

N° de cat : 86201-1-PBS

Synonymes

NKCC1, SLC12A2, Basolateral Na K Cl symporter, Basolateral Na-K-Cl symporter, BSC

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Galerie de données de validation

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  WB analysis using 86201-1-RR (same clone as 86201-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86201-1-RR (NKCC1/SLC12A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86201-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 86201-1-RR (same clone as 86201-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86201-1-RR (NKCC1/SLC12A2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86201-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 86201-1-RR (same clone as 86201-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86201-1-RR (NKCC1/SLC12A2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86201-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 86201-1-RR (same clone as 86201-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86201-1-RR (NKCC1/SLC12A2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86201-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 86201-1-RR (same clone as 86201-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86201-1-RR (NKCC1/SLC12A2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86201-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86201-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86201-1-RR against Human NKCC1/SLC12A2 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

86201-1-PBS cible NKCC1/SLC12A2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NKCC1/SLC12A2 Protéine recombinante Ag4991
• Nom complet: solute carrier family 12 (sodium/potassium/chloride transporters), member 2
• Masse moléculaire calculée: 131 kDa
• Poids moléculaire observé: 150-160 kDa
• Numéro d’acquisition GenBank: BC033003
• Symbole du gène: NKCC1/SLC12A2
• Identification du gène (NCBI): 6558
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The Na-K-Cl cotransporter sodium-potassium-chloride cotransporter 1 (NKCC1), encoded by the SLC12A2 gene, is an intensively studied member of the CCC family. NKCC1 is a membrane protein and plays fundamental roles in regulating trans-epithelial ion movement, cell volume, chloride homeostasis and neuronal excitability. NKCC1 assembles into a dimer, with the first ten transmembrane (TM) helices harboring the transport core and TM11-TM12 helices lining the dimer interface(PMID: 32081947). The observed molecular weight of NKCC1 is about 140 kDa, glycosylated NKCC1 is detected a band at ∼170 kDa (PMID: 22723696).

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