Anticorps Polyclonal de lapin anti-NKD2

• Phare
• Validé par KD/KO

NKD2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 16699-1-AP

Synonymes

Naked-2, Naked2, Naked 2, hNkd2

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Galerie de données de validation

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  WB analysis of mouse kidney using 16699-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16699-1-AP (NKD2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16699-1-AP

  WB result of NKD2 antibody (16699-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-NKD2 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 16699-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16699-1-AP (NKD2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 16699-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 16699-1-AP (NKD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HEK-293, tissu de côlon de souris

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

16699-1-AP cible NKD2 dans les applications de WB, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NKD2 Protéine recombinante Ag10094
• Nom complet: naked cuticle homolog 2 (Drosophila)
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC012176
• Symbole du gène: NKD2
• Identification du gène (NCBI): 85409
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Naked2 (protein naked cuticle homolog 2), encoded by NKD2 gene, was identified as a cell autonomous antagonist of the canonical Wnt signaling pathway. Naked2 acts as a cargo recognition and targeting protein to ensure proper delivery and fusion of TGF-alpha-containing vesicles to a distinct region at the basolateral surface of polarized epithelial cells (PMID: 17553928). EGFR-independent action of TGF-alpha protects Naked2 from proteasomal degradation, thus ensuring its delivery to the basolateral surface (PMID: 18757723). Myristoylated Naked2 antagonizes Wnt-beta-catenin activity possibly by degrading Dishevelled-1 at the plasma membrane (PMID: 20177058).

Publications

Species	Application	Title
human	WB	Oncogene; NKD2, a negative regulator of Wnt signaling, suppresses tumor growth and metastasis in osteosarcoma.; Authors - S Zhao ; KD Validated
human	WB	Biosci Biotechnol Biochem; Silencing of lncRNA ZFAS1 inhibits malignancies by blocking Wnt/β-catenin signaling in gastric cancer cells.; Authors - Weiran Xu
human	WB	Kaohsiung J Med Sci; Curcumin suppresses the Wnt/β-catenin signaling pathway by inhibiting NKD2 methylation to ameliorate intestinal ischemia/reperfusion injury; Authors - Jia-Xi Zhu
human	WB,IP,CoIP	Rejuvenation Res; Tripartite Motif-Containing Protein 65 Promotes Proliferation and Inhibits Ferroptosis in Prostate Cancer via Enhancing NKD Inhibitor of WNT Signaling Pathway 2 Ubiquitination; Authors - Chengcai Wang

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