Anticorps Polyclonal de lapin anti-NKIRAS2

NKIRAS2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

Avis (2)

N° de cat : 10174-1-AP

Synonymes

I-kappa-B-interacting Ras-like protein 2, Kappa B Ras protein 2, Kappa B-Ras protein 2, kappaB Ras2, KappaB-Ras2

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Galerie de données de validation

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  WB analysis of HEK-293 using 10174-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10174-1-AP (NKIRAS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10174-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10174-1-AP (NKIRAS2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  IP experiment of HEK-293 using 10174-1-AP

  IP result of anti-NKIRAS2 (IP:10174-1-AP, 4ug; Detection:10174-1-AP 1:300) with HEK-293 cells lysate 2400 ug.

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  IF Staining of HeLa using 10174-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NKIRAS2 antibody (10174-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

10174-1-AP cible NKIRAS2 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NKIRAS2 Protéine recombinante Ag0221
• Nom complet: NFKB inhibitor interacting Ras-like 2
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC007450
• Symbole du gène: NKIRAS2
• Identification du gène (NCBI): 28511
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

I-Kappa-B-interacting Ras-like protein 2 (KBRAS2, MGC:4553), interact with the PEST domains of IkappaBalpha and IkappaBbeta [inhibitors of the transcription factor nuclear factor kappa B (NF-kappaB)] and decrease their rate of degradation. KBRAS2 differs from other Ras proteins in containing Ala and Leu residues at positions 12 and 61 respectively, which are similar to those present in the oncogenic forms of Ras.

Publications

Species	Application	Title
human	WB	Nat Commun; κB-Ras and Ral GTPases regulate acinar to ductal metaplasia during pancreatic adenocarcinoma development and pancreatitis.; Authors - Stephanie Beel
human	WB	Nat Commun; Characterization of dsRNA binding proteins through solubility analysis identifies ZNF385A as a dsRNA homeostasis regulator; Authors - Na Jiang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Rebecca (Verified Customer) (06-13-2025)

The antibody works very well for western blot. I used it at 1:1000 dilution in 3% BSA in TBST (overnight incubation).

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

NKIRAS2 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:10174-1-AP)

FH

Andrea (Verified Customer) (09-18-2020)

The antibody works great in Western Blot. Good signal and specificity, which was tested on knockout cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: pancreas, different human and mouse cell lines

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