Anticorps Polyclonal de lapin anti-NKX2-5

• Phare
• Validé par KD/KO

NKX2-5 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

Avis (1)

N° de cat : 13921-1-AP

Synonymes

Cardiac specific homeobox, CHNG5, CSX, CSX1, Homeobox protein CSX, Homeobox protein Nkx 2.5, NKX2 5, NKX2.5, NKX2-5, NKX2E, NKX4 1

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Galerie de données de validation

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  WB analysis of human heart using 13921-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13921-1-AP (NKX2-5 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13921-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13921-1-AP (NKX2-5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 13921-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13921-1-AP (NKX2-5 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse heart using 13921-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13921-1-AP (NKX2-5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 13921-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13921-1-AP (NKX2-5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 13921-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13921-1-AP (NKX2-5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of mouse embryo using 13921-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using NKX2-5 antibody (13921-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque humain
• Résultats positifs en IHC: tissu cérébral humain, tissu cardiaque de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu embryonnaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IF: See 3 publications below

Informations sur le produit

13921-1-AP cible NKX2-5 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NKX2-5 Protéine recombinante Ag4930
• Nom complet: NK2 transcription factor related, locus 5 (Drosophila)
• Masse moléculaire calculée: 324 aa, 35 kDa
• Poids moléculaire observé: 30-42 kda
• Numéro d’acquisition GenBank: BC025711
• Symbole du gène: NKX2-5
• Identification du gène (NCBI): 1482
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development [PMID: 7665173]. NKX2-5 encoded by homeobox-containing genes that implicated in commitment to and/or differentiation of the myocardial lineage, and acts as a transcriptional activator of ANF in cooperation with GATA4. During embryonic development, NKX2-5 plays pivotal roles in specifying cardiac progenitors, cardiac morphogenesis, cardiomyocyte differentiation and conduction system development [PMID: 22824467].

Publications

Species	Application	Title
human	WB	Cell Rep; Post-transcriptional Regulation of Nkx2-5 by RHAU in Heart Development.; Authors - Junwei Nie
human	IF	iScience; A dual SHOX2:GFP; MYH6:mCherry knockin hESC reporter line for derivation of human SAN-like cells.; Authors - Zaniar Ghazizadeh
rat	WB	Am J Physiol Heart Circ Physiol; Homocysteine downregulates cardiac homeobox transcription factor NKX2.5 via IGFBP5.; Authors - Guangrui Lai
mouse	WB	J Appl Toxicol; The effect of maternal exposure to di-(2-ethylhexyl)-phthalate on fetal cardiac development in mice.; Authors - Changqing Tang
rat	WB	Mol Med Rep; H3K9me2 regulates early transcription factors to promote mesenchymal stem‑cell differentiation into cardiomyocytes.; Authors - Xiaolin Sun
rat	IF	Int J Clin Exp Pathol; SDF-1α promotes repair of myocardial ischemic necrosis zones in rats.; Authors - Xinhua Cai

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Rebecca (Verified Customer) (10-25-2022)

20ug of protein were loaded. Transference was performed at 4ºC and 90V for 2h. Antibody incubation was done ON at 4ºC.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: iPSC-CM

NKX2-5 Antibody Western Blot validation (1:1000 dilution) in iPSC-CM (Cat no:13921-1-AP)