Anticorps Polyclonal de lapin anti-NKX2-5

• Phare
• Validé par KD/KO

NKX2-5 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13921-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of human heart using 13921-1-AP (same clone as 13921-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 13921-1-AP (NKX2-5 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13921-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 13921-1-AP (same clone as 13921-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 13921-1-AP (NKX2-5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13921-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 13921-1-AP (same clone as 13921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 13921-1-AP (NKX2-5 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13921-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 13921-1-AP (same clone as 13921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13921-1-AP (NKX2-5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13921-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 13921-1-AP (same clone as 13921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13921-1-AP (NKX2-5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13921-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 13921-1-AP (same clone as 13921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 13921-1-AP (NKX2-5 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13921-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse embryo using 13921-1-AP (same clone as 13921-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using NKX2-5 antibody (13921-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13921-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13921-1-PBS cible NKX2-5 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NKX2-5 Protéine recombinante Ag4930
• Nom complet: NK2 transcription factor related, locus 5 (Drosophila)
• Masse moléculaire calculée: 324 aa, 35 kDa
• Poids moléculaire observé: 30-42 kda
• Numéro d’acquisition GenBank: BC025711
• Symbole du gène: NKX2-5
• Identification du gène (NCBI): 1482
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development [PMID: 7665173]. NKX2-5 encoded by homeobox-containing genes that implicated in commitment to and/or differentiation of the myocardial lineage, and acts as a transcriptional activator of ANF in cooperation with GATA4. During embryonic development, NKX2-5 plays pivotal roles in specifying cardiac progenitors, cardiac morphogenesis, cardiomyocyte differentiation and conduction system development [PMID: 22824467].

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