Anticorps Polyclonal de lapin anti-NKX3-1

NKX3-1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

Avis (1)

N° de cat : 13069-1-AP

Synonymes

BAPX2, Homeobox protein Nkx 3.1, NK3 homeobox 1, NKX3, NKX3 1, NKX3.1, NKX3-1, NKX3A

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Galerie de données de validation

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  WB analysis of LNCaP using 13069-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 13069-1-AP (NKX3-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 13069-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13069-1-AP (NKX3-1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 13069-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13069-1-AP (NKX3-1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 13069-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13069-1-AP (NKX3-1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, tissu testiculaire humain
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 2 publications below

Informations sur le produit

13069-1-AP cible NKX3-1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NKX3-1 Protéine recombinante Ag4070
• Nom complet: NK3 homeobox 1
• Masse moléculaire calculée: 234 aa, 26 kDa
• Poids moléculaire observé: 34-38 kDa
• Numéro d’acquisition GenBank: BC074864
• Symbole du gène: NKX3-1
• Identification du gène (NCBI): 4824
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NKX3-1, also named as NKX3.1 and NKX3A, belongs to the NK-3 homeobox family. It is a transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. NKX3-1 plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. It act as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells(PMID:19462257). The calcualted molecular weight of NKX3-1 is 26 kDa, but modified NKX3-1 is about 32-35 kDa. (PMID: 18597829)

Publications

Species	Application	Title
human	IHC	bioRxiv; ERG activates a stem-like proliferation-differentiation program in prostate epithelial cells with mixed basal-luminal identity; Authors - Weiran Feng
mouse	IHC	Sci Rep; Prostatic androgen receptor signaling shows an age-related and lobe-specific alteration in mice; Authors - Han Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Parmveer (Verified Customer) (08-28-2019)

Worked well for both immunohistochemistry and immunofluorescence. FFPE prostate tissue used, primary applied for 1 hour at room temperature. There is a bit of background that is hard to avoid, but luminal prostate nuclei are clearly marked.

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Prostate

NKX3-1 Antibody Immunohistochemistry,Immunofluorescence, validation (1:500 dilution) in Prostate (Cat no:13069-1-AP)

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