Anticorps Recombinant de lapin anti-NKX3-1

NKX3-1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250402B8

N° de cat : 86085-1-RR

Synonymes

BAPX2, Homeobox protein NK-3 homolog A, Homeobox protein Nkx 3.1, Homeobox protein Nkx-3.1, NK3 homeobox 1

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Galerie de données de validation

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  WB analysis of LNCaP using 86085-1-RR

  LNCaP cells were subjected to SDS PAGE followed by western blot with 86085-1-RR (NKX3-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86085-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86085-1-RR against Human NKX3-1 were performed. The affinity constant is 0.411 nM.

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Applications testées

• Résultats positifs en WB: cellules LNCaP,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86085-1-RR cible NKX3-1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NKX3-1 Protéine recombinante Ag4070
• Nom complet: NK3 homeobox 1
• Masse moléculaire calculée: 234 aa, 26 kDa
• Poids moléculaire observé: 34-38 kDa
• Numéro d’acquisition GenBank: BC074864
• Symbole du gène: NKX3-1
• Identification du gène (NCBI): 4824
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NKX3-1, also named as NKX3.1 and NKX3A, belongs to the NK-3 homeobox family. It is a transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. NKX3-1 plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. It act as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells(PMID:19462257). The calcualted molecular weight of NKX3-1 is 26 kDa, but modified NKX3-1 is about 32-35 kDa. (PMID: 18597829)

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