Anticorps Recombinant de lapin anti-NKp46/NCR1

NKp46/NCR1 Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241811H7

N° de cat : 84416-5-PBS

Synonymes

241811H7, Activating receptor 1, Ar1, CD335, Kilr1

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Galerie de données de validation

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  WB analysis of mouse splenocytes using 84416-5-RR (same clone as 84416-5-PBS)

  mouse splenocytes were subjected to SDS PAGE followed by western blot with 84416-5-RR (NKp46/NCR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84416-5-PBS in a different storage buffer formulation.

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  IHC staining of rat spleen using 84416-5-RR (same clone as 84416-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84416-5-RR (NKp46/NCR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84416-5-PBS in a different storage buffer formulation.

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  IHC staining of rat spleen using 84416-5-RR (same clone as 84416-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84416-5-RR (NKp46/NCR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84416-5-PBS in a different storage buffer formulation.

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  IHC staining of rat thymus using 84416-5-RR (same clone as 84416-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 84416-5-RR (NKp46/NCR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84416-5-PBS in a different storage buffer formulation.

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  IF Staining of rat spleen using 84416-5-RR (same clone as 84416-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using NKp46/NCR1 antibody (84416-5-RR, Clone: 241811H7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84416-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84416-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84416-5-RR against Rat NKp46/NCR1 were performed. The affinity constant is 0.593 nM.

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Informations sur le produit

84416-5-PBS cible NKp46/NCR1 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NKp46/NCR1 Protéine recombinante Eg1469
• Nom complet: natural cytotoxicity triggering receptor 1
• Masse moléculaire calculée: 37kDa
• Poids moléculaire observé: 50-60 kDa
• Numéro d’acquisition GenBank: NM_057199.1
• Symbole du gène: NKp46/NCR1
• Identification du gène (NCBI): 117547
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NKp46, also known as NCR1 or CD335, is an Ig-like superfamily cell surface receptor that is highly conserved in mammals (PMID: 22021440). NKp46 is a type I transmembrane glycoprotein consisting of two extracellular Ig-like domains, a transmembrane domain, and an intracellular tail (PMID: 9730896). It is expressed on NK cells, rare T-cell subsets, and a mucosal population of NKp46+ innate lymphoid cells (PMID: 9730896; 22021440). NKp46 is the major triggering receptor involved in the natural cytotoxicity (PMID: 10359120; 10092106; 15356098).

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