Anticorps Monoclonal anti-NLGN1

NLGN1 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

CloneNo.

2D1A12

Avis (1)

N° de cat : 66964-1-Ig

Synonymes

KIAA1070, neuroligin 1, NLGN1

---

Galerie de données de validation

• 

  WB analysis of mouse brain using 66964-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66964-1-Ig (NLGN1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66964-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66964-1-Ig (NLGN1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit brain using 66964-1-Ig

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 66964-1-Ig (NLGN1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 66964-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66964-1-Ig (NLGN1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66964-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66964-1-Ig (NLGN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 66964-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66964-1-Ig (NLGN1 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66964-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66964-1-Ig (NLGN1 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de lapin, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral humain, tissu de cervelet de porc, tissu de cervelet de rat, tissu de cervelet de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochimie (IHC): IHC : 1:750-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below

Informations sur le produit

66964-1-Ig cible NLGN1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NLGN1 Protéine recombinante Ag6356
• Nom complet: neuroligin 1
• Masse moléculaire calculée: 840 aa, 94 kDa
• Poids moléculaire observé: 116 kDa
• Numéro d’acquisition GenBank: BC032555
• Symbole du gène: NLGN1
• Identification du gène (NCBI): 22871
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NLGN1, also named KIAA1070, belongs to the type-B carboxylesterase/lipase family. Neuroligin1 (NLGN1) is a main component of the excitatory glutamatergic synapses complex and plays a role in synapse assembly and function (PMID: 34340665). NLGN1 has 2 isoforms with the MV of 92 and 96 kDa. Sometimes higher molecular weight of around 116 kDa can also be observed, which is a glycosylated mature form of NLGN1 (PMID: 28841651).

Publications

Species	Application	Title
mouse	WB	Neuron; Longitudinal single-cell transcriptional dynamics throughout neurodegeneration in SCA1; Authors - Leon Tejwani
rat	WB	Neuroscience; Activity-dependent Synaptic Recruitment of Neuroligin 1 in Spinal Dorsal Horn Contributed to Inflammatory Pain.; Authors - Ji-Yuan Zhao
mouse	WB	J Ethnopharmacol; Multimodal investigation reveals the neuroprotective mechanism of Angong Niuhuang pill for intracerebral hemorrhage: Converging bioinformatics, network pharmacology, and experimental validation; Authors - Jiawei Zhou
human	WB	Aging (Albany NY); Metastasis-related gene signature associates with immunity and predicts prognosis accurately in patients with osteosarcoma; Authors - Sen Qin
mouse	WB	Neurochem Int; Mitochondrial protein prohibitin promotes learning memory recovery in mice following intracerebral hemorrhage via CAMKII/CRMP signaling pathway; Authors - Tianlin Jiang
mouse	WB	Aging (Albany NY); Longitudinal characterization of behavioral, morphological and transcriptomic changes in a tauopathy mouse model; Authors - Qing Cao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Wojciech (Verified Customer) (02-08-2023)

We used this antibody to assess protein levels in human T-ALL cells by western blot. Loaded 30 ug of protein. We got a good signal with 1:5000 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: T cells