Anticorps Recombinant de lapin anti-NLRC5

NLRC5 Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240338D8

N° de cat : 83384-2-PBS

Synonymes

240338D8, Caterpiller protein 16.1, CLR16.1, NOD27, NOD4

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Galerie de données de validation

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  IF Staining of THP-1 using 83384-2-RR (same clone as 83384-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using NLRC5 antibody (83384-2-RR, Clone: 240338D8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83384-2-PBS in a different storage buffer formulation.

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  FC experiment of THP-1 using 83384-2-RR (same clone as 83384-2-PBS)

  1x10^6 THP-1 cells were intracellularly stained with 0.25 ug NLRC5 Recombinant antibody (83384-2-RR, Clone:240338D8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83384-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00402-3

  Cytometric bead array standard curve of MP00402-3, NLRC5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83384-2-PBS. Detection antibody: 83384-3-PBS. Standard: Ag34508. Range: 1.56-100 ng/mL.

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  Affinity and Kinetic Characterization of 83384-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83384-2-RR against Human NLRC5 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83384-2-PBS cible NLRC5 dans les applications de IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NLRC5 Protéine recombinante Ag34508
• Nom complet: NLR family, CARD domain containing 5
• Masse moléculaire calculée: 204 kDa
• Numéro d’acquisition GenBank: BC063566
• Symbole du gène: NLRC5
• Identification du gène (NCBI): 84166
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The nucleotide-binding domain leucine-rich repeat containing (NLR) family of proteins is mainly involved in microbial pathogen recognition, inflammatory responses, and cell death. NLRC5, the largest member of the NLR family, is currently receiving an increasing level of attention. NLRC5 plays a role in cytokine response and antiviral immunity through its inhibition of NF-kappa-B activation and negative regulation of type I interferon signaling pathways.

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