Anticorps Polyclonal de lapin anti-NLRP1

NLRP1 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

Avis (1)

N° de cat : 12256-1-AP

Synonymes

CARD7, CLR17.1, DEFCAP, DEFCAP L/S, KIAA0926, NAC, NALP1, NLRP1, PP1044, SLEV1, VAMAS1

---

Galerie de données de validation

• 

  IHC staining of mouse brain using 12256-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12256-1-AP (NLRP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12256-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12256-1-AP (NLRP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12256-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12256-1-AP (NLRP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 12256-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12256-1-AP (NLRP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12256-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12256-1-AP (NLRP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 12256-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12256-1-AP (NLRP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12256-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12256-1-AP (NLRP1 antibody) at dilution of 1:50 (under 10x lens).

---

Applications testées

• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral de rat, tissu de cancer du pancréas humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

12256-1-AP cible NLRP1 dans les applications de IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NLRP1 Protéine recombinante Ag2908
• Nom complet: NLR family, pyrin domain containing 1
• Masse moléculaire calculée: 155 kDa
• Numéro d’acquisition GenBank: BC016965
• Symbole du gène: NLRP1
• Identification du gène (NCBI): 22861
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human		MBio; Inducible Nitric Oxide Synthase Is a Key Host Factor for Toxoplasma GRA15-Dependent Disruption of the Gamma Interferon-Induced Antiparasitic Human Response.; Authors - Hironori Bando
human	IF	Mol Neurodegener; The NLRP3 and NLRP1 inflammasomes are activated in Alzheimer's disease.; Authors - Marina Saresella
human	IHC	Front Immunol; Cytokine Secretion and Pyroptosis of Thyroid Follicular Cells Mediated by Enhanced NLRP3, NLRP1, NLRC4, and AIM2 Inflammasomes Are Associated With Autoimmune Thyroiditis.; Authors - Qingling Guo
human	IHC	Front Microbiol; Porphyromonas gingivalis-Induced NLRP3 Inflammasome Activation and Its Downstream Interleukin-1β Release Depend on Caspase-4.; Authors - Pei-Hui Ding
human	IF	Neuroscience; Overexpression of MicroRNA-9a-5p Ameliorates NLRP1 Inflammasome-mediated Ischemic Injury in Rats Following Ischemic Stroke.; Authors - Cao Yuze
human		Inflammation; Aspergillus fumigatus-Stimulated Human Corneal Epithelial Cells Induce Pyroptosis of THP-1 Macrophages by Secreting TSLP.; Authors - Qingshan Ji

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Debanjan (Verified Customer) (08-17-2020)

The band was very clear and of exact molecular weight

• Applications: Western Blot
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: Human fibroblasts