Anticorps Polyclonal de lapin anti-NLRP12

NLRP12 Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30998-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 30998-1-AP (same clone as 30998-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30998-1-AP (NLRP12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30998-1-PBS in a different storage buffer formulation.

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  IP experiment of rat liver using 30998-1-AP (same clone as 30998-1-PBS)

  IP result of anti-NLRP12 (IP:30998-1-AP, 4ug; Detection:30998-1-AP 1:500) with rat liver tissue lysate 2695 ug. This data was developed using the same antibody clone with 30998-1-PBS in a different storage buffer formulation.

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  IP experiment of PC-3 using 30998-1-AP (same clone as 30998-1-PBS)

  IP result of anti-NLRP12 (IP:30998-1-AP, 4ug; Detection:30998-1-AP 1:500) with PC-3 cells lysate 1575 ug. This data was developed using the same antibody clone with 30998-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30998-1-PBS cible NLRP12 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NLRP12 Protéine recombinante Ag34281
• Nom complet: NLR family, pyrin domain containing 12
• Masse moléculaire calculée: 1061 aa, 120 kDa
• Poids moléculaire observé: 95-110 kDa
• Numéro d’acquisition GenBank: BC028069
• Symbole du gène: NLRP12
• Identification du gène (NCBI): 91662
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NACHT, LRR and PYD domains-containing protein 12 (NLRP12) is also named as Monarch-1, NALP12, PYPAF7 and RNO. NLRP12 is as an essential cytosolic sensor for heme plus PAMPs-mediated PANoptosis, inflammation, and pathology (PMID: 37267949). NLRP12 drives inflammasome and PANoptosome activation, cell death, and inflammation in response to heme plus PAMPs or TNF. TLR2/4-mediated signaling through IRF1 induced Nlrp12 expression, which led to inflammasome formation to induce maturation of IL-1β and IL-18 (PMID: 37267949). NLRP12, a pyrin-containing NLR protein, is a negative regulator of innate immune activation and type I interferon (IFN-I) production (PMID: 36719379).

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