Anticorps Recombinant de lapin anti-NLRP3

NLRP3 Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250083C8

N° de cat : 85967-1-PBS

Synonymes

AGTAVPRL, AII/AVP, Mmig1, MWS, NALP3

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Galerie de données de validation

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  WB analysis using 85967-1-RR (same clone as 85967-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85967-1-RR (NLRP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85967-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 85967-1-RR (same clone as 85967-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85967-1-RR (NLRP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85967-1-PBS in a different storage buffer formulation.

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Informations sur le produit

85967-1-PBS cible NLRP3 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NLRP3 Protéine recombinante Ag32515
• Nom complet: NLR family, pyrin domain containing 3
• Masse moléculaire calculée: 118kd
• Poids moléculaire observé: 108-120 kDa
• Numéro d’acquisition GenBank: NM_145827
• Symbole du gène: Nlrp3
• Identification du gène (NCBI): 216799
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NLRP3, also named as NALP3, CIASI, C1orf7, PYPAF1, Cryopyrin and MIM606416, belongs to NLR family. NLRP3, a key and eponymous component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. NLRP3 inflammasome is expressed in immune cells, including monocytes, macrophages, and dendritic cells. When the NLRP3 inflammasome is activated, the PYD domain of NLRP3 mediates recruitment of an adaptor protein called ASC and the effector protein procaspase-1 to form an NLRP3 inflammasome complex that can cleave inactive procaspase-1 to from active caspase-1. And then the active caspase-1 results in secretion of interleukin (IL)-18 and IL-1β. Activation of the NLRP3 inflammasome is also required for HMGB1 secretion. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death (PMID:27669650). NLRP3 has some isoforms with the MW of 106-118 kDa and 75-83 kDa. An additional 130-135 kDa band could also be detected (PMID: 17164409; PMID: 34680443; PMID: 35585627). This antibody is specific for mouse NLRP3.

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