Anticorps Recombinant de lapin anti-NLRP6

NLRP6 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240543B2

N° de cat : 83555-5-RR

Synonymes

PYPAF5, NALP6, 240543B2

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Galerie de données de validation

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  WB analysis of mouse colon using 83555-5-RR

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 83555-5-RR (NLRP6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 83555-5-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using NLRP6 antibody (83555-5-RR, Clone: 240543B2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  Affinity and Kinetic Characterization of 83555-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83555-5-RR against Human NLRP6 were performed. The affinity constant is 3.65 nM.

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Applications testées

• Résultats positifs en WB: tissu de côlon de souris,
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83555-5-RR cible NLRP6 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NLRP6 Protéine recombinante Ag34516
• Nom complet: NLR family, pyrin domain containing 6
• Masse moléculaire calculée: 99 kDa
• Poids moléculaire observé: 98 kDa
• Numéro d’acquisition GenBank: NM_138329.2
• Symbole du gène: NLRP6
• Identification du gène (NCBI): 171389
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NOD-like receptor family pyrin domain containing 6 (NLRP6) is also named as NACHT, LRR and PYD domains-containing protein 6 and ZFML, Angiotensin II/vasopressin receptor, NALP6 and PYPAF5. NLRP6 forms a complex with ASC and caspase-1 or caspase-11 to form an inflammasome complex cleaving pro-interleukin-1β (IL-1β) and IL-18 into their biologically active forms. The function of NLRP6 has been attributed to the maintenance of epithelial integrity and host defense against microbial infections (PMID: 33910012). NLRP6 is reported to involved in inflammasome activation, regulation of nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling, antiviral interferon (IFN) signaling, mucus secretion, and antimicrobial peptide (AMP) production. NLRP6 is a novel NLR family member, that shows high expression in the intestine and liver (in contrast to NLRP3 in myeloid cells), to regulate inflammation and host defense against microbes (PMID: 32386845).

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