Anticorps Polyclonal de lapin anti-NMDAR1/GRIN1

NMDAR1/GRIN1 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27232-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 27232-1-AP (same clone as 27232-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27232-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27232-1-PBS in a different storage buffer formulation.

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  IP experiment of rat brain using 27232-1-AP (same clone as 27232-1-PBS)

  IP result of anti-NMDAR1/GRIN1 (IP:27232-1-AP, 4ug; Detection:27232-1-AP 1:400) with rat brain tissue lysate 1120 ug. This data was developed using the same antibody clone with 27232-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 27232-1-AP (same clone as 27232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27232-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27232-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 27232-1-AP (same clone as 27232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27232-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27232-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 27232-1-AP (same clone as 27232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27232-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27232-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 27232-1-AP (same clone as 27232-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using NMDAR1/GRIN1 antibody (27232-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27232-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 27232-1-AP (same clone as 27232-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using NMDAR1/GRIN1 antibody (27232-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27232-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27232-1-PBS cible NMDAR1/GRIN1 dans les applications de WB, IHC, IF-P, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NMDAR1/GRIN1 Protéine recombinante Ag26093
• Nom complet: glutamate receptor, ionotropic, N-methyl D-aspartate 1
• Masse moléculaire calculée: 105 kDa
• Poids moléculaire observé: 116-120 kDa
• Numéro d’acquisition GenBank: NM_000832
• Symbole du gène: NMDAR1
• Identification du gène (NCBI): 2902
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GRIN1 encodes subunit 1 of the N-methyl-D-aspartate (NMDA) receptor, which is a heteromeric glutamate-gated calcium ion channel essential for synaptic function in the brain (PMID: 25864721, PMID: 25864721). NMDARs play important roles in normal brain development and function, such as synaptic plasticity, neural development, learning and memory (PMID: 20716669). NMDAR dysfunction has been associated with several neurological disorders including Parkinson, Alzheimer and Huntington diseases. Disrupted motor learning and long-term synaptic plasticity in mice lacking NMDAR1 in the striatum (PMID: 17015831).

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