Anticorps Recombinant de lapin anti-NMDAR1/GRIN1

NMDAR1/GRIN1 Recombinant Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

250535B1

N° de cat : 85973-3-RR

Synonymes

GRIN1, NMDAR1, GluN1, Glutamate receptor ionotropic, NMDA 1, NMDAR 1

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Galerie de données de validation

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  WB analysis using 85973-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85973-3-RR (NMDAR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 85973-3-RR

  IP result of anti-NMDAR1/GRIN1 (IP:85973-3-RR, 4ug; Detection:85973-3-RR 1:1000) with mouse brain tissue lysate 1720 ug.

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  Affinity and Kinetic Characterization of 85973-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85973-3-RR against Human NMDAR1/GRIN1 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85973-3-RR cible NMDAR1/GRIN1 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NMDAR1/GRIN1 Protéine recombinante Ag26093
• Nom complet: glutamate receptor, ionotropic, N-methyl D-aspartate 1
• Masse moléculaire calculée: 105 kDa
• Poids moléculaire observé: 116-120 kDa
• Numéro d’acquisition GenBank: NM_000832
• Symbole du gène: NMDAR1
• Identification du gène (NCBI): 2902
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GRIN1 encodes subunit 1 of the N-methyl-D-aspartate (NMDA) receptor, which is a heteromeric glutamate-gated calcium ion channel essential for synaptic function in the brain (PMID: 25864721, PMID: 25864721). NMDARs play important roles in normal brain development and function, such as synaptic plasticity, neural development, learning and memory (PMID: 20716669). NMDAR dysfunction has been associated with several neurological disorders including Parkinson, Alzheimer and Huntington diseases. Disrupted motor learning and long-term synaptic plasticity in mice lacking NMDAR1 in the striatum (PMID: 17015831).

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