Anticorps Polyclonal de lapin anti-NMDAR2A/GRIN2A

NMDAR2A/GRIN2A Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, IF-P, IF-Fro, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 28571-1-AP

Synonymes

GRIN2A, NMDAR2A, glun2a, Glutamate [NMDA] receptor subunit epsilon-1, Glutamate receptor ionotropic, NMDA 2A

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Galerie de données de validation

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  WB analysis using 28571-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28571-1-AP (NMDAR2A/GRIN2A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 28571-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28571-1-AP (NMDAR2A/GRIN2A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 28571-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28571-1-AP (NMDAR2A/GRIN2A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 28571-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28571-1-AP (NMDAR2A/GRIN2A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 28571-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28571-1-AP (NMDAR2A/GRIN2A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 28571-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NMDAR2A/GRIN2A antibody (28571-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 28571-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NMDAR2A/GRIN2A antibody (28571-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of rat brain using 28571-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using NMDAR2A/GRIN2A antibody (28571-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cervelet de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en IF-Fro: tissu cérébral de rat,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

28571-1-AP cible NMDAR2A/GRIN2A dans les applications de WB, IHC, IF-P, IF-Fro, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NMDAR2A/GRIN2A Protéine recombinante Ag29423
• Nom complet: glutamate receptor, ionotropic, N-methyl D-aspartate 2A
• Masse moléculaire calculée: 165 kDa
• Poids moléculaire observé: 180 kDa
• Numéro d’acquisition GenBank: NM_000833
• Symbole du gène: GRIN2A
• Identification du gène (NCBI): 2903
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Behav Brain Res; Down-regulation of NR2B receptors contributes to the analgesic and antianxiety effects of enriched environment mediated by endocannabinoid system in the inflammatory pain mice; Authors - Shukun Jiang
rat	WB	Heliyon; Cherry leaf decoction inhibits NMDAR expression and thereby ameliorates CUMS- induced depression-like behaviors through downregulation of α2δ-1; Authors - Chuan Jiang
mouse	WB	Neurochem Res; Scavenger Receptor Class B Type I Modulates Epileptic Seizures and Receptor α2δ-1 Expression; Authors - Yunyi Huang
mouse	WB	J Ethnopharmacol; Anticonvulsant effect of Stachydrine on pentylenetetrazole-induced kindling seizure mouse model via Notch and NMDAR signaling pathways; Authors - Qifa Li
mouse	IF	Neuron; The paraventricular thalamus mediates visceral pain and anxiety-like behaviors via two distinct pathways; Authors - Di Li

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