Anticorps Recombinant de lapin anti-NMDAR2A/GRIN2A

NMDAR2A/GRIN2A Recombinant Antibody for WB, IF-P, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240445D4

N° de cat : 83465-2-RR

Synonymes

GRIN2A, NMDAR2A, hNR2A, Glutamate receptor ionotropic, NMDA 2A, Glutamate [NMDA] receptor subunit epsilon-1

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Galerie de données de validation

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  WB analysis using 83465-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83465-2-RR (GRIN2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of rat brain using 83465-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NMDAR2A/GRIN2A antibody (83465-2-RR, Clone: 240445D4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 83465-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NMDAR2A/GRIN2A antibody (83465-2-RR, Clone: 240445D4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of U-251 using 83465-2-RR

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug Grin2a Recombinant Antibody (83465-2-RR, Clone:240445D4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83465-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83465-2-RR against Human NMDAR2A/GRIN2A were performed. The affinity constant is 11.0 nM.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IF-P: tissu cérébral de rat,
• Résultats positifs en FC (Intra): cellules U-251,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83465-2-RR cible NMDAR2A/GRIN2A dans les applications de WB, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NMDAR2A/GRIN2A Protéine recombinante Ag29101
• Nom complet: glutamate receptor, ionotropic, N-methyl D-aspartate 2A
• Masse moléculaire calculée: 165 kDa
• Poids moléculaire observé: 160-180 kDa
• Numéro d’acquisition GenBank: NM_000833
• Symbole du gène: GRIN2A
• Identification du gène (NCBI): 2903
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GRIN2A (glutamate ionotropic receptor NMDA type subunit 2A), also known as NMDAR2A. And its molecular weight is 165 kDa. GRIN2A is located in cell projection, dendritic spine, cell membrane, synapse, postsynaptic cell membrae, cytolamic vesicle membrane, which is expressed in many tissues, highest expression in brain and heart. This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without cognitive disability. Alternative splicing results in multiple transcript variants.

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