Anticorps Recombinant de lapin anti-NME1

NME1 Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242670D5

N° de cat : 85038-7-PBS

Synonymes

242670D5, AWD, EC:2.7.4.6, GAAD, Granzyme A activated DNase

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Galerie de données de validation

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  WB analysis using 85038-7-RR (same clone as 85038-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85038-7-RR (NME1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85038-7-PBS in a different storage buffer formulation.

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  WB analysis using 85038-7-RR (same clone as 85038-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85038-7-RR (NME1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85038-7-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 85038-7-RR (same clone as 85038-7-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NME1 antibody (85038-7-RR, Clone: 242670D5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85038-7-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 85038-7-RR (same clone as 85038-7-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NME1 Recombinant antibody (85038-7-RR, Clone:242670D5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85038-7-PBS in a different storage buffer formulation.

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Informations sur le produit

85038-7-PBS cible NME1 dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NME1 Protéine recombinante Ag1548
• Nom complet: non-metastatic cells 1, protein (NM23A) expressed in
• Masse moléculaire calculée: 17 kDa
• Poids moléculaire observé: 16-20 kDa
• Numéro d’acquisition GenBank: BC018994
• Symbole du gène: NME1
• Identification du gène (NCBI): 4830
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NME1 is also named as NDPKA(Nucleoside diphosphate kinase A), NM23(Metastasis inhibition factor nm23),GAAD(Granzyme A-activated DNase) and belongs to the NDK family.It is a major role in the synthesis of nucleoside triphosphates other than ATP and required for neural development including neural patterning and cell fate determination. It has 3 isoforms produced by alternative splicing. Refer to the epitope and protein detection, this antibody specifically recognizes NME1.

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