Anticorps Polyclonal de lapin anti-NME2

NME2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20493-1-PBS

Synonymes

C-myc purine-binding transcription factor PUF, EC:2.7.13.3, EC:2.7.4.6, Histidine protein kinase NDKB, NDKB

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Galerie de données de validation

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  WB analysis of mouse kidney using 20493-1-AP (same clone as 20493-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20493-1-AP (NME2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20493-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 20493-1-AP (same clone as 20493-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20493-1-AP (NME2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20493-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 20493-1-AP (same clone as 20493-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20493-1-AP (NME2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20493-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 20493-1-AP (same clone as 20493-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20493-1-AP (NME2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20493-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 20493-1-AP (same clone as 20493-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 20493-1-AP (NME2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20493-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20493-1-PBS cible NME2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NME2 Protéine recombinante Ag13913
• Nom complet: non-metastatic cells 2, protein (NM23B) expressed in
• Masse moléculaire calculée: 152 aa, 17 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC002476
• Symbole du gène: NME2
• Identification du gène (NCBI): 4831
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NME2(non-metastatic cells 2, protein) is also named as NDKB(Nucleoside diphosphate kinase B) and NM23B, and belongs to the NDK family. It is involved in the phosphorylation of nucleoside diphosphates, supplying nucleotide pools for DNA/RNA synthesis, and interacts with membrane receptors and constitutes a novel molecular regulatory mechanism of GPCR endocytosis. This protein has 2 isoforms produced by alternative splicing.

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