Anticorps Recombinant de lapin anti-NME3

NME3 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241585H7

N° de cat : 84278-5-PBS

Synonymes

241585H7, DR nm23, DR-nm23, EC:2.7.4.6, KIAA0516

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Galerie de données de validation

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  WB analysis using 84278-5-RR (same clone as 84278-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84278-5-RR (NME3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84278-5-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 84278-5-RR (same clone as 84278-5-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 84278-5-RR (NME3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84278-5-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 84278-5-RR (same clone as 84278-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 84278-5-RR (NME3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84278-5-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 84278-5-RR (same clone as 84278-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 84278-5-RR (NME3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84278-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84278-5-RR (same clone as 84278-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NME3 antibody (84278-5-RR, Clone: 241585H7 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 84278-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84278-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84278-5-RR against Human NME3 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84278-5-PBS cible NME3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NME3 Protéine recombinante Ag7284
• Nom complet: non-metastatic cells 3, protein expressed in
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC000250
• Symbole du gène: NME3
• Identification du gène (NCBI): 4832
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NME3 (NME/NM23 Nucleoside Diphosphate Kinase 3), also named as NM23-H3; DR-NM23, is a member of the nucleoside diphosphate kinase (NDPK) family that binds to the mitochondrial outer membrane to stimulate mitochondrial fusion. The depletion of NME3 will cause dysfunction in mitochondrial dynamics. NME3 has been found to facilitate DNA-repair mechanisms binds to several NPHPs, including NEK8, CEP164, and ankyrin repeat and sterile α motif domain-containing 6 (ANKS6)

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