Anticorps Polyclonal de lapin anti-NME5

NME5 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 12923-1-AP

Synonymes

IPIA beta, NDK H 5, NDP kinase homolog 5, NME5, Testis specific nm23 homolog

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Galerie de données de validation

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  WB analysis of mouse testis using 12923-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12923-1-AP (NME5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 12923-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12923-1-AP (NME5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12923-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12923-1-AP (NME5 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 12923-1-AP

  IP result of anti-NME5 (IP:12923-1-AP, 4ug; Detection:12923-1-AP 1:500) with mouse testis tissue lysate 3920 ug.

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  IHC staining of mouse testis using 12923-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12923-1-AP (NME5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 12923-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12923-1-AP (NME5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, tissu rénal humain
• Résultats positifs en IP: tissu testiculaire de souris,
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

12923-1-AP cible NME5 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NME5 Protéine recombinante Ag3610
• Nom complet: non-metastatic cells 5, protein expressed in (nucleoside-diphosphate kinase)
• Masse moléculaire calculée: 212 aa, 24 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC026182
• Symbole du gène: NME5
• Identification du gène (NCBI): 8382
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NME5(Nucleoside diphosphate kinase homolog 5) belongs to the NDK family. Its expression plays a critical role in spermiogenesis by increasing the cellular levels of GPX5 to eliminate reactive oxygen species. NME5 may have specific functions in the phosphotransfer network involved in spermiogenesis and flagellar movement.

Publications

Species	Application	Title
mouse	WB,IF	Curr Biol; CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement; Authors - Chao Liu
mouse	WB	J Hazard Mater; TCDD promotes liver fibrosis through disordering systemic and hepatic iron homeostasis.; Authors - Changying Li
human	IHC	Int J Cancer; Additive effect of the AZGP1, PIP, S100A8, and UBE2C molecular biomarkers improves outcome prediction in breast carcinoma.; Authors - Parris Toshima Z TZ
mouse	WB,IF	Cell Rep; Differential requirements of IQUB for the assembly of radial spoke 1 and the motility of mouse cilia and flagella; Authors - Xin Zhang
mouse	WB	Cell Mol Life Sci; CCDC146 is required for sperm flagellum biogenesis and male fertility in mice; Authors - Yanjie Ma