Anticorps Recombinant de lapin anti-NMI

• Phare
• Validé par KD/KO

NMI Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241135A9

N° de cat : 83986-2-RR

Synonymes

241135A9, N myc (and STAT) interactor, N myc and STAT interactor, N myc interactor, N-myc and STAT interactor

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Galerie de données de validation

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  WB analysis of HeLa using 83986-2-RR

  WB result of NMI antibody (83986-2-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NMI transfected HeLa cells.

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  WB analysis using 83986-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83986-2-RR (NMI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 83986-2-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NMI antibody (83986-2-RR, Clone: 241135A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HepG2 using 83986-2-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NMI Recombinant antibody (83986-2-RR, Clone:241135A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83986-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83986-2-RR against Human NMI were performed. The affinity constant is 1.98 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules K-562, cellules PC-3
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83986-2-RR cible NMI dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NMI Protéine recombinante Ag34759
• Nom complet: N-myc (and STAT) interactor
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC001268
• Symbole du gène: NMI
• Identification du gène (NCBI): 9111
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

N-Myc and STAT Interactor protein (NMI) is an interferon inducible protein that interacts with NMYC and CMYC (members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. It is widely involved in the process of tumor growth, progression, and metastasis.

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