Anticorps Polyclonal de lapin anti-NMNAT1

• Phare
• Validé par KD/KO

NMNAT1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 11399-1-AP

Synonymes

NaMN adenylyltransferase 1, NMNAT1, PNAT 1, PNAT1

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Galerie de données de validation

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  WB analysis of human testis using 11399-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11399-1-AP (NMNAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 11399-1-AP

  pig heart tissue were subjected to SDS PAGE followed by western blot with 11399-1-AP (NMNAT1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11399-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11399-1-AP (NMNAT1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 11399-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11399-1-AP (NMNAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 11399-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11399-1-AP (NMNAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain, tissu cardiaque de porc, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de muscle squelettique de souris, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

11399-1-AP cible NMNAT1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NMNAT1 Protéine recombinante Ag1944
• Nom complet: nicotinamide nucleotide adenylyltransferase 1
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 28-32 kDa
• Numéro d’acquisition GenBank: BC014943
• Symbole du gène: NMNAT1
• Identification du gène (NCBI): 64802
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NMNAT1 is a member of the nicotinamide-nucleotide adenylyltransferases (NMNATs) which catalyze nicotinamide adenine dinucleotide (NAD) synthesis (PMID: 28445802). NMNAT is a central enzyme in NAD biosynthesis, catalyzing the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP (PMID: 17402747). NMNAT1 is widely expressed with the highest levels in skeletal muscle, heart, and kidney(PMID: 11027696). Mutations in NMNAT1 have been shown associated with the LCA9 form of the retinal degeneration pathology Leber's congenital amaurosis (PMID: 22842229, 22842230).

Publications

Species	Application	Title
mouse	IHC	J Am Soc Nephrol; Pre-emptive Short-term Nicotinamide Mononucleotide Treatment in a Mouse Model of Diabetic Nephropathy.; Authors - Itaru Yasuda
human,mouse	IF	Metabolism; Hyperglycemia-reduced NAD+ biosynthesis impairs corneal epithelial wound healing in diabetic mice.; Authors - Ya Li
human	WB	J Proteome Res; Reduced Nicotinamide Mononucleotide (NMNH) Potently Enhances NAD(+) and Suppresses Glycolysis, the TCA Cycle, and Cell Growth; Authors - Yan Liu
mouse	IHC	Sci Rep; Nicotinamide mononucleotide ameliorates adriamycin-induced renal damage by epigenetically suppressing the NMN/NAD consumers mediated by Twist2; Authors - Kazuhiro Hasegawa

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Caitlin (Verified Customer) (09-17-2020)

I used this antibody on neuronal cell lysate for Western blotting. The antibody was able to detect the protein of interest in my lysate (NMNAT3).

• Applications: Western Blot
• Cell Tissue Type: Mouse neurons