Anticorps Polyclonal de lapin anti-NMT1

• Phare
• Validé par KD/KO

NMT1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 11546-1-AP

Synonymes

N myristoyltransferase 1, NMT1, Type I N myristoyltransferase

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Galerie de données de validation

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  WB analysis of SKOV-3 using 11546-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11546-1-AP

  WB result of NMT1 antibody (11546-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NMT1 transfected HeLa cells.

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  WB analysis of human kidney using 11546-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 11546-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11546-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 11546-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 11546-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11546-1-AP

  IP Result of anti-NMT1 (IP:11546-1-AP, 4ug; Detection:11546-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human gliomas using 11546-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11546-1-AP (NMT1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 11546-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11546-1-AP (NMT1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 11546-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11546-1-AP (NMT1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 11546-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11546-1-AP (NMT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11546-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11546-1-AP (NMT1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 11546-1-AP

  Immunofluorescent analysis of MCF-7 cells, using NMT1 antibody 11546-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules SKOV-3, cellules HeLa, cellules L02, cellules PC-3, tissu pancréatique de souris, tissu rénal humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de gliome humain, tissu cardiaque humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

11546-1-AP cible NMT1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NMT1 Protéine recombinante Ag2072
• Nom complet: N-myristoyltransferase 1
• Masse moléculaire calculée: 496 aa, 57 kDa
• Poids moléculaire observé: 49-68 kDa
• Numéro d’acquisition GenBank: BC006569
• Symbole du gène: NMT1
• Identification du gène (NCBI): 4836
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NMT1 is a N-myristoyltransferase responsible for the transfer of myristate from CoA to an amino-terminal glycine of many eukaryotic proteins, which facilitates the targeting of proteins to membrane surfaces and is essential for viability of the organism. Insertional mutagenesis of the Nmt1 gene in Saccharomyces cerevisiae causes recessive lethality. Humans and mice possess two distinct but structurally similar enzymes, NMT1 and NMT2, ubiquitously expressed in most human and mouse tissues. Western analysis revealed that there are 4 isoforms of NMT1 with apparent molecular masses ranging from 49 to 68 kDa. In cell fractionation studies, the 68-kDa NMT1 isoform and NMT2 were present in both membrane and cytoplasmic fractions, while the smaller NMT1 isoforms were predominantly cytoplasmic.

Publications

Species	Application	Title
human	WB	Brain; Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA.; Authors - Janja Božič
human	WB,IF	Cancer Lett; N-myristoyltransferase-1 deficiency blocks myristoylation of LAMTOR1 and inhibits bladder cancer progression.; Authors - Yi Sun ; KD Validated
human	WB,IHC	Cell Death Dis; NMT1 inhibition modulates breast cancer progression through stress-triggered JNK pathway.; Authors - Lu Deng ; KD Validated
human	WB	FASEB J; Regulation of co- and post-translational myristoylation of proteins during apoptosis: interplay of N-myristoyltransferases and caspases.; Authors - Perinpanayagam Maneka A MA
mouse	WB	Biochem Biophys Res Commun; N-myristoyltransferase 1 interacts with calnexin at the endoplasmic reticulum.; Authors - Elzbieta Dudek
human		Oncotarget; Tris DBA palladium is highly effective against growth and metastasis of pancreatic cancer in an orthotopic model.; Authors - Begoña Díaz