- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-NNMT
NNMT Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Conjugaison
Non conjugué
N° de cat : 15123-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules L02, cellules HepG2, tissu hépatique de rat, tissu hépatique de souris | 
| Résultats positifs en IP | cellules HepG2 | 
| Résultats positifs en IHC | tissu de cancer de l'estomac humain, il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. | 
| Résultats positifs en IF/ICC | cellules HepG2 | 
| Résultats positifs en FC (Intra) | cellules HepG2, | 
Dilution recommandée
| Application | Dilution | 
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 | 
| Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate | 
| Immunohistochimie (IHC) | IHC : 1:400-1:1600 | 
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 | 
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| KD/KO | See 7 publications below | 
| WB | See 22 publications below | 
| IHC | See 8 publications below | 
| IF | See 2 publications below | 
| IP | See 1 publications below | 
Informations sur le produit
15123-1-AP cible NNMT dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
| Réactivité | Humain, rat, souris | 
| Réactivité citée | rat, Humain, souris | 
| Hôte / Isotype | Lapin / IgG | 
| Clonalité | Polyclonal | 
| Type | Anticorps | 
| Immunogène | NNMT Protéine recombinante Ag7197 | 
| Nom complet | nicotinamide N-methyltransferase | 
| Masse moléculaire calculée | 30 kDa | 
| Poids moléculaire observé | 30 kDa | 
| Numéro d’acquisition GenBank | BC000234 | 
| Symbole du gène | NNMT | 
| Identification du gène (NCBI) | 4837 | 
| Conjugaison | Non conjugué | 
| Forme | Liquide | 
| Méthode de purification | Purification par affinité contre l'antigène | 
| Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol | 
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. | 
Informations générales
NNMT can catalyze the N-methylation of nicotinamide using the universal methyl donor S-adenosyl-L-methionine to form N1-methylnicotinamide and S-adenosyl-L-homocysteine, a predominant nicotinamide/vitamin B3 clearance pathway (PMID: 21823666; 23455543; 8182091). It plays a central role in regulating cellular methylation potential, by consuming S-adenosyl-L-methionine and limiting its availability for other methyltransferases. Actively mediates genome-wide epigenetic and transcriptional changes through hypomethylation of repressive chromatin marks, such as H3K27me3 (PMID: 23455543; PMID: 26571212; PMID: 31043742).
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for NNMT antibody 15123-1-AP | Download protocol | 
| IHC protocol for NNMT antibody 15123-1-AP | Download protocol | 
| IF protocol for NNMT antibody 15123-1-AP | Download protocol | 
| IP protocol for NNMT antibody 15123-1-AP | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 
Publications
| Species | Application | Title | 
|---|---|---|
| Cell Rep Single-cell analysis reveals insights into epithelial abnormalities in ovarian endometriosis | ||
| Oncogene Stromal nicotinamide N-methyltransferase orchestrates the crosstalk between fibroblasts and tumour cells in oral squamous cell carcinoma: evidence from patient-derived assembled organoids | ||
| Mol Metab Disrupted Liver Oxidative Metabolism in Glycine N-Methyltransferase-Deficient Mice is Mitigated by Dietary Methionine Restriction. | ||
| Mol Oncol Elevated N-methyltransferase expression induced by hepatic stellate cells contributes to the metastasis of hepatocellular carcinoma via regulation of the CD44v3 isoform.
 | ||
| Front Cell Infect Microbiol Persistent Exposure to Porphyromonas gingivalis Promotes Proliferative and Invasion Capabilities, and Tumorigenic Properties of Human Immortalized Oral Epithelial Cells. | ||
| Sci Rep The significance of NAD + metabolites and nicotinamide N-methyltransferase in chronic kidney disease. | 
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Kenzo (Verified Customer) (01-05-2024) | This antibody worked for immunofluorescence with a little background 
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| FH Johnny (Verified Customer) (01-07-2022) | products from proteintech have always been reliable. 
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