Anticorps Polyclonal de lapin anti-NNT

• Phare
• Validé par KD/KO

NNT Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 13442-2-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of human adrenal gland using 13442-2-AP (same clone as 13442-2-PBS)

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 13442-2-AP (NNT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13442-2-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 13442-2-AP (same clone as 13442-2-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 13442-2-AP (NNT antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13442-2-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 13442-2-AP (same clone as 13442-2-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 13442-2-AP (NNT antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13442-2-PBS in a different storage buffer formulation.

• 

  WB analysis of human adrenal gland using 13442-2-AP (same clone as 13442-2-PBS)

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 13442-2-AP (NNT antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13442-2-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 13442-2-AP (same clone as 13442-2-PBS)

  IP result of anti-NNT (IP:13442-2-AP, 4ug; Detection:13442-2-AP 1:300) with HepG2 cells lysate 2800ug. This data was developed using the same antibody clone with 13442-2-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 13442-2-AP (same clone as 13442-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13442-2-AP (NNT antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13442-2-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 13442-2-AP (same clone as 13442-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13442-2-AP (NNT antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13442-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13442-2-AP (same clone as 13442-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NNT antibody (13442-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13442-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13442-2-AP (same clone as 13442-2-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13442-2-AP (NNT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13442-2-PBS in a different storage buffer formulation.

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Informations sur le produit

13442-2-PBS cible NNT dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NNT Protéine recombinante Ag4259
• Nom complet: nicotinamide nucleotide transhydrogenase
• Masse moléculaire calculée: 1085 aa, 114 kDa
• Poids moléculaire observé: 114 kDa
• Numéro d’acquisition GenBank: BC032370
• Symbole du gène: NNT
• Identification du gène (NCBI): 23530
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NNT(nicotinamide nucleotide transhydrogenase) is a transmembrane protein and functions as a proton pumping transhydrogenase. The protein is present in both prokaryotes and eukaryotes and is located in the inner membrane of mitochondria. In prokaryotic cells, the enzyme is composed of α and βsubunits of 54 and 48 kDa, respectively. In eukaryotic cells, the enzyme is usually composed of a single peptide of 110 kDa. Although NNT catalyzes the interconversion of NADH and NADPH, the forward reaction using the reducing power of NADH to regenerate NADPH would be favored under conditions of oxidative stress(PMID:16497723). It can exsit as a homodimer(PMID:21882037).

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