Anticorps Monoclonal anti-NNT

NNT Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F5H6

N° de cat : 68194-1-PBS

Synonymes

NNT

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Galerie de données de validation

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  WB analysis of LNCaP using 68194-1-Ig (same clone as 68194-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68194-1-Ig (NNT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

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  WB analysis using 68194-1-Ig (same clone as 68194-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68194-1-Ig (NNT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

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  WB analysis using 68194-1-Ig (same clone as 68194-1-PBS)

  Various lysates (mouse heart tissue and mouse liver tissue were dissected from BALB/c mice) were subjected to SDS PAGE followed by western blot with 68194-1-Ig (NNT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 68194-1-Ig (same clone as 68194-1-PBS)

  IP result of anti-NNT (IP:68194-1-Ig, 4ug; Detection:68194-1-Ig 1:5000) with HepG2 cells lysate 960 ug. This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 68194-1-Ig (same clone as 68194-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68194-1-Ig (NNT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 68194-1-Ig (same clone as 68194-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68194-1-Ig (NNT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 68194-1-Ig (same clone as 68194-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68194-1-Ig (NNT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 68194-1-Ig (same clone as 68194-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68194-1-Ig (NNT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68194-1-Ig (same clone as 68194-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NNT antibody (68194-1-Ig, Clone: 1F5H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 68194-1-Ig (same clone as 68194-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NNT antibody (68194-1-Ig, Clone: 1F5H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68194-1-PBS cible NNT dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NNT Protéine recombinante Ag4259
• Nom complet: nicotinamide nucleotide transhydrogenase
• Masse moléculaire calculée: 1085 aa, 114 kDa
• Poids moléculaire observé: 114 kDa
• Numéro d’acquisition GenBank: BC032370
• Symbole du gène: NNT
• Identification du gène (NCBI): 23530
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NNT(nicotinamide nucleotide transhydrogenase) is a transmembrane protein and functions as a proton pumping transhydrogenase. The protein is present in both prokaryotes and eukaryotes and is located in the inner membrane of mitochondria. In prokaryotic cells, the enzyme is composed of α and β subunits of 54 and 48 kDa, respectively. In eukaryotic cells, the enzyme is usually composed of a single peptide of 114 kDa. Although NNT catalyzes the interconversion of NADH and NADPH, the forward reaction using the reducing power of NADH to regenerate NADPH would be favored under conditions of oxidative stress(PMID:16497723). It can exsit as a homodimer(PMID:21882037).

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