Anticorps Polyclonal de lapin anti-NO66/C14orf169

NO66/C14orf169 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 22842-1-AP

Synonymes

C14orf169, hsNO66, MAPJD, NO66, NO66/C14orf169, Nucleolar protein 66

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Galerie de données de validation

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  WB analysis using 22842-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22842-1-AP (NO66/C14orf169 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 22842-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22842-1-AP (NO66/C14orf169 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 22842-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 22842-1-AP (NO66/C14orf169 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 22842-1-AP

  IP result of anti-NO66/C14orf169 (IP:22842-1-AP, 4ug; Detection:22842-1-AP 1:500) with Jurkat cells lysate 4000ug.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules Jurkat, cellules NCCIT
• Résultats positifs en IP: cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

22842-1-AP cible NO66/C14orf169 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NO66/C14orf169 Protéine recombinante Ag18721
• Nom complet: chromosome 14 open reading frame 169
• Masse moléculaire calculée: 641 aa, 71 kDa
• Poids moléculaire observé: 71 kDa
• Numéro d’acquisition GenBank: BC011350
• Symbole du gène: NO66
• Identification du gène (NCBI): 79697
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NO66, also known as C14orf169 and MAPJD, is a JmjC domain-containing oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. NO66 is highly conserved during evolution and shows a dual localization pattern, present in both the nucleoplasm and nucleolus (PMID: 14742713). It has been reported that NO66 participates in regulation of Osx transcriptional activity and plays an important role in osteoblast differentiation through interaction with Osx (PMID: 19927124; 23620590). Through interaction with MYC protein, NO66 plays a role in pulmonary carcinogenesis (PMID: 17308053).

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