Anticorps Monoclonal anti-NOB1

NOB1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

8C11A12

N° de cat : 66048-1-PBS

Synonymes

ART 4, ART4, MST158, MSTP158, NOB1, NOB1P, Protein ART 4, PSMD8BP1, RNA binding protein NOB1

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Galerie de données de validation

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  WB analysis of Jurkat using 66048-1-Ig (same clone as 66048-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66048-1-Ig (NOB1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66048-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 66048-1-Ig (same clone as 66048-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66048-1-Ig (NOB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66048-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66048-1-Ig (same clone as 66048-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66048-1-Ig (NOB1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66048-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66048-1-Ig (same clone as 66048-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66048-1-Ig (NOB1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66048-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66048-1-PBS cible NOB1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NOB1 Protéine recombinante Ag17907
• Nom complet: NIN1/RPN12 binding protein 1 homolog (S. cerevisiae)
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC000050
• Symbole du gène: NOB1
• Identification du gène (NCBI): 28987
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NOB1 was first identified in yeast as an essential gene encoding the Nin one binding protein, which is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA. In addition, NOB1 is a crucial molecule in the maturation of the 20S proteasome and protein degradation. It serves as a chaperone to join the 20S proteasome with the 19S regulatory particle in the nucleus and facilitates the maturation of the 20S proteasome. Recently NOB1 has been reported to be overexpressed in several types of cancer, suggesting its involvement in the tumorigenesis.

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