Anticorps Polyclonal de lapin anti-NOD2

• Phare
• Validé par KD/KO

NOD2 Polyclonal Antibody for IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(17)

N° de cat : 20980-1-AP

Synonymes

Nucleotide-binding oligomerization domain-containing protein 2, Caspase recruitment domain-containing protein 15, CARD15

---

Galerie de données de validation

• 

  IHC staining of human colon using 20980-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20980-1-AP (NOD2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 20980-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20980-1-AP (NOD2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 20980-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 20980-1-AP (NOD2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining using 20980-1-AP

  IF result of 20980-1-AP from publication with PMID: 31186034.

• 

  IF Staining using 20980-1-AP

  IF result of 20980-1-AP from publication with PMID: 31186034.

• 

  IF Staining using 20980-1-AP

  IF result of 20980-1-AP from publication with PMID: 31186034.

---

Applications testées

• Résultats positifs en IHC: tissu de côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IHC: See 8 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

20980-1-AP cible NOD2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: nucleotide-binding oligomerization domain containing 2
• Masse moléculaire calculée: 115 kDa
• Numéro d’acquisition GenBank: NM_022162
• Symbole du gène: NOD2
• Identification du gène (NCBI): 64127
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NOD2, also named as CARD15 and IBD1, induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK-gamma. It confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Defects in NOD2 are the cause of Blau syndrome (BS). Defects in NOD2 are a cause of susceptibility to Crohn disease (CD). Defects in NOD2 are a cause of susceptibility to ulcerative colitis. Defects in NOD2 are the cause of early-onset sarcoidosis (EOS). The antibody is specific to NOD2.

Publications

Species	Application	Title
mouse	WB,IHC	Kidney Int; PMID: 23594678; Authors - Du Pengchao P
mouse	WB	Kidney Int; Progranulin protects against renal ischemia/reperfusion injury in mice.; Authors - Meng Zhou
mouse	WB	Hypertension; Novel Role of NOD2 in Mediating Ca2+ Signaling: Evidence From NOD2-Regulated Podocyte TRPC6 Channels in Hyperhomocysteinemia.; Authors - Han Huirong H
mouse	WB,IF	J Neuroinflammation; NOD2 promotes dopaminergic degeneration regulated by NADPH oxidase 2 in 6-hydroxydopamine model of Parkinson's disease.; Authors - Li Cheng
rat, human	WB, IHC	Free Radic Biol Med; Identification of NOD2 as a novel target of RNA-binding protein HuR:Evidence from NADPH oxidase-mediated HuR signaling in diabetic nephropathy.; Authors - Jin Shang
human	WB	Biochim Biophys Acta Mol Cell Biol Lipids; Effects of peptidoglycan on the development of steatohepatitis.; Authors - Meiling Jin

{{ptg:RelatedPrimaryAntibodies}}