Anticorps Monoclonal anti-NOD2

NOD2 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(4)

CloneNo.

1A10D1

N° de cat : 66710-1-Ig

Synonymes

ACUG, BLAU, CARD15, CD, CLR16.3, IBD1, NLRC2, NOD2, NOD2B, PSORAS1

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Galerie de données de validation

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  WB analysis using 66710-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66710-1-Ig (NOD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules K-562, cellules Raji, cellules THP-1

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

66710-1-Ig cible NOD2 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: nucleotide-binding oligomerization domain containing 2
• Masse moléculaire calculée: 115 kDa
• Numéro d’acquisition GenBank: NM_022162
• Symbole du gène: NOD2
• Identification du gène (NCBI): 64127
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

OD2, also named as CARD15 and IBD1, induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK-gamma. It confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Defects in NOD2 are the cause of Blau syndrome (BS). Defects in NOD2 are a cause of susceptibility to Crohn disease (CD). Defects in NOD2 are a cause of susceptibility to ulcerative colitis. Defects in NOD2 are the cause of early-onset sarcoidosis (EOS).

Publications

Species	Application	Title
	WB	Front Pharmacol; CircEpc1 Promotes Ricin Toxin-Induced Inflammation via Activation of NF-κB and MAPK Signaling Pathways by Sponging miR-5114.; Authors - Mingxin Dong
	WB,IF	Front Cell Infect Microbiol; Extracellular Histone H3 Induces Pyroptosis During Sepsis and May Act Through NOD2 and VSIG4/NLRP3 Pathways.; Authors - Chun-Xia Shi
	WB	J Cell Mol Med; β-arrestin 2 negatively regulates NOD2 signalling pathway through association with TRAF6 in microglia after cerebral ischaemia/reperfusion injury.; Authors - Lin Chen
human	WB	Front Pharmacol; Postbiotic muramyl dipeptide alleviates colitis via activating autophagy in intestinal epithelial cells; Authors - Yaying You

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