Anticorps Recombinant de lapin anti-NOD2

NOD2 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230327E10

N° de cat : 83281-3-RR

Synonymes

230327E10, CARD15, Caspase recruitment domain-containing protein 15, Nucleotide-binding oligomerization domain-containing protein 2

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Galerie de données de validation

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  IF Staining of HeLa using 83281-3-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NOD2 antibody (83281-3-RR, Clone: 230327E10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of Jurkat using 83281-3-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Nod2 Recombinant Antibody (83281-3-RR, Clone:230327E10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83281-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83281-3-RR against Human NOD2 were performed. The affinity constant is 3.79 nM.

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Applications testées

• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83281-3-RR cible NOD2 dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NOD2 Protéine recombinante Ag33771
• Nom complet: nucleotide-binding oligomerization domain containing 2
• Masse moléculaire calculée: 115 kDa
• Numéro d’acquisition GenBank: NM_001293557
• Symbole du gène: NOD2
• Identification du gène (NCBI): 64127
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NOD2, also named as CARD15 and IBD1, induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK-gamma. It confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Defects in NOD2 are the cause of Blau syndrome (BS). Defects in NOD2 are a cause of susceptibility to Crohn disease (CD). Defects in NOD2 are a cause of susceptibility to ulcerative colitis. Defects in NOD2 are the cause of early-onset sarcoidosis (EOS).

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