Anticorps Recombinant de lapin anti-NOL11

NOL11 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240371B5

N° de cat : 83391-6-RR

Synonymes

Nucleolar protein 11, L14, 240371B5

---

Galerie de données de validation

• 

  WB analysis using 83391-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83391-6-RR (NOL11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 83391-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NOL11 antibody (83391-6-RR, Clone: 240371B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 83391-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NOL11 antibody (83391-6-RR, Clone: 240371B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 83391-6-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug NOL11 Recombinant antibody (83391-6-RR, Clone:240371B5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  Affinity and Kinetic Characterization of 83391-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83391-6-RR against Human NOL11 were performed. The affinity constant is below 1 pM.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules HepG2, cellules HT-29, cellules SW480
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83391-6-RR cible NOL11 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NOL11 Protéine recombinante Ag11783
• Nom complet: nucleolar protein 11
• Masse moléculaire calculée: 719 aa, 81 kDa
• Poids moléculaire observé: 81 kDa
• Numéro d’acquisition GenBank: BC064404
• Symbole du gène: NOL11
• Identification du gène (NCBI): 25926
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NOL11, also named as L14, is a ribosome biogenesis factor. It is required for both optimal rDNA transcription and small subunit (SSU) pre-rRNA processing at sites A', A0, 1 and 2b. NOL11 forms a protein complex with two tryptophan-aspartic acid (WD) repeat proteins named WD-repeat protein 43 (WDR43) and Cirhin in mitotic cells. NOL11 plays a role in pathogenesis of NAIC. There're some isoforms with MW 81 kDa and 19 kDa.

{{ptg:RelatedPrimaryAntibodies}}