Anticorps Polyclonal de lapin anti-NOL12

• Phare
• Validé par KD/KO

NOL12 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15456-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis using 15456-1-AP (same clone as 15456-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15456-1-AP (NOL12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15456-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 15456-1-AP (same clone as 15456-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15456-1-AP (NOL12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15456-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 15456-1-AP (same clone as 15456-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15456-1-AP (NOL12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15456-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 15456-1-AP (same clone as 15456-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15456-1-AP (NOL12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15456-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 15456-1-AP (same clone as 15456-1-PBS)

  IP result of anti-NOL12 (IP:15456-1-AP, 4ug; Detection:15456-1-AP 1:300) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 15456-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 15456-1-AP (same clone as 15456-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NOL12 antibody (15456-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15456-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

15456-1-PBS cible NOL12 dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NOL12 Protéine recombinante Ag7723
• Nom complet: nucleolar protein 12
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC002808
• Symbole du gène: NOL12
• Identification du gène (NCBI): 79159
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Nucleolar protein 12 (NOL12), a multifunctional RNA-binding protein, has been found to be related to genomic stability, DNA damage repair, and apoptosis. High expression of NOL12 is associated with worse reduced overall survival (OS), high pathological grade, node metastasis, and advanced clinical stage in patients with HCC (PMID: 35693698). Knockdown of NOL12 impairs cellular proliferation by inducing a G1/S cell cycle arrest, but does so outside of a nucleolar stress response and in a p53-independent manner (PMID: 29069457).

{{ptg:RelatedPrimaryAntibodies}}